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2.Mechanically pulverize the sample into a mush. For example, slice or grind the sample. 3.Transfer pulverized sample to a sterile tube and mix well with 100uL (or other volume that can immerse the sample) of Direct DNA Solution. 4.Incubate overnight at 37C in an incubator. 5.Spin the tube at maximum speed for 10 min.
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